SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum.
Identifieur interne : 003031 ( Main/Exploration ); précédent : 003030; suivant : 003032SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum.
Auteurs : Kèvin Knoops [Pays-Bas] ; Marjolein Kikkert ; Sjoerd H E Van Den Worm ; Jessika C. Zevenhoven-Dobbe ; Yvonne Van Der Meer ; Abraham J. Koster ; A Mieke Mommaas ; Eric J. SnijderSource :
- PLoS biology [ 1545-7885 ] ; 2008.
Descripteurs français
- KwdFr :
- ARN double brin (génétique), ARN double brin (métabolisme), ARN viral (génétique), ARN viral (métabolisme), Animaux, Cryomicroscopie électronique, Humains, Imagerie tridimensionnelle, Immunohistochimie, Lignée cellulaire, Membranes intracellulaires (métabolisme), Membranes intracellulaires (ultrastructure), Modèles biologiques, Protéines virales (génétique), Protéines virales (métabolisme), RNA replicase (génétique), RNA replicase (métabolisme), Réplication virale, Réticulum endoplasmique (métabolisme), Réticulum endoplasmique (ultrastructure), Sous-unités de protéines (génétique), Sous-unités de protéines (métabolisme), Structures macromoléculaires (métabolisme), Virus du SRAS (physiologie), Virus du SRAS (ultrastructure).
- MESH :
- génétique : ARN double brin, ARN viral, Protéines virales, RNA replicase, Sous-unités de protéines.
- métabolisme : ARN double brin, ARN viral, Membranes intracellulaires, Protéines virales, RNA replicase, Réticulum endoplasmique, Sous-unités de protéines, Structures macromoléculaires.
- physiologie : Virus du SRAS.
- ultrastructure : Animaux, Cryomicroscopie électronique, Humains, Imagerie tridimensionnelle, Immunohistochimie, Lignée cellulaire, Membranes intracellulaires, Modèles biologiques, Réplication virale, Réticulum endoplasmique, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Cell Line, Cryoelectron Microscopy, Endoplasmic Reticulum (metabolism), Endoplasmic Reticulum (ultrastructure), Humans, Imaging, Three-Dimensional, Immunohistochemistry, Intracellular Membranes (metabolism), Intracellular Membranes (ultrastructure), Macromolecular Substances (metabolism), Models, Biological, Protein Subunits (genetics), Protein Subunits (metabolism), RNA Replicase (genetics), RNA Replicase (metabolism), RNA, Double-Stranded (genetics), RNA, Double-Stranded (metabolism), RNA, Viral (genetics), RNA, Viral (metabolism), SARS Virus (physiology), SARS Virus (ultrastructure), Viral Proteins (genetics), Viral Proteins (metabolism), Virus Replication.
- MESH :
- chemical , genetics : Protein Subunits, RNA Replicase, RNA, Double-Stranded, RNA, Viral, Viral Proteins.
- chemical , metabolism : Macromolecular Substances, Protein Subunits, RNA Replicase, RNA, Double-Stranded, RNA, Viral, Viral Proteins.
- metabolism : Endoplasmic Reticulum, Intracellular Membranes.
- physiology : SARS Virus.
- ultrastructure : Endoplasmic Reticulum, Intracellular Membranes, SARS Virus.
- Animals, Cell Line, Cryoelectron Microscopy, Humans, Imaging, Three-Dimensional, Immunohistochemistry, Models, Biological, Virus Replication.
Abstract
Positive-strand RNA viruses, a large group including human pathogens such as SARS-coronavirus (SARS-CoV), replicate in the cytoplasm of infected host cells. Their replication complexes are commonly associated with modified host cell membranes. Membrane structures supporting viral RNA synthesis range from distinct spherular membrane invaginations to more elaborate webs of packed membranes and vesicles. Generally, their ultrastructure, morphogenesis, and exact role in viral replication remain to be defined. Poorly characterized double-membrane vesicles (DMVs) were previously implicated in SARS-CoV RNA synthesis. We have now applied electron tomography of cryofixed infected cells for the three-dimensional imaging of coronavirus-induced membrane alterations at high resolution. Our analysis defines a unique reticulovesicular network of modified endoplasmic reticulum that integrates convoluted membranes, numerous interconnected DMVs (diameter 200-300 nm), and "vesicle packets" apparently arising from DMV merger. The convoluted membranes were most abundantly immunolabeled for viral replicase subunits. However, double-stranded RNA, presumably revealing the site of viral RNA synthesis, mainly localized to the DMV interior. Since we could not discern a connection between DMV interior and cytosol, our analysis raises several questions about the mechanism of DMV formation and the actual site of SARS-CoV RNA synthesis. Our data document the extensive virus-induced reorganization of host cell membranes into a network that is used to organize viral replication and possibly hide replicating RNA from antiviral defense mechanisms. Together with biochemical studies of the viral enzyme complex, our ultrastructural description of this "replication network" will aid to further dissect the early stages of the coronavirus life cycle and its virus-host interactions.
DOI: 10.1371/journal.pbio.0060226
PubMed: 18798692
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001A66
- to stream PubMed, to step Curation: 001A66
- to stream PubMed, to step Checkpoint: 001A32
- to stream Ncbi, to step Merge: 001D49
- to stream Ncbi, to step Curation: 001D49
- to stream Ncbi, to step Checkpoint: 001D49
- to stream Main, to step Merge: 003104
- to stream Main, to step Curation: 003031
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum.</title><author><name sortKey="Knoops, Kevin" sort="Knoops, Kevin" uniqKey="Knoops K" first="Kèvin" last="Knoops">Kèvin Knoops</name><affiliation wicri:level="3"><nlm:affiliation>Section Electron Microscopy, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Section Electron Microscopy, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Kikkert, Marjolein" sort="Kikkert, Marjolein" uniqKey="Kikkert M" first="Marjolein" last="Kikkert">Marjolein Kikkert</name></author><author><name sortKey="Worm, Sjoerd H E Van Den" sort="Worm, Sjoerd H E Van Den" uniqKey="Worm S" first="Sjoerd H E Van Den" last="Worm">Sjoerd H E Van Den Worm</name></author><author><name sortKey="Zevenhoven Dobbe, Jessika C" sort="Zevenhoven Dobbe, Jessika C" uniqKey="Zevenhoven Dobbe J" first="Jessika C" last="Zevenhoven-Dobbe">Jessika C. Zevenhoven-Dobbe</name></author><author><name sortKey="Van Der Meer, Yvonne" sort="Van Der Meer, Yvonne" uniqKey="Van Der Meer Y" first="Yvonne" last="Van Der Meer">Yvonne Van Der Meer</name></author><author><name sortKey="Koster, Abraham J" sort="Koster, Abraham J" uniqKey="Koster A" first="Abraham J" last="Koster">Abraham J. Koster</name></author><author><name sortKey="Mommaas, A Mieke" sort="Mommaas, A Mieke" uniqKey="Mommaas A" first="A Mieke" last="Mommaas">A Mieke Mommaas</name></author><author><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J" last="Snijder">Eric J. Snijder</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18798692</idno><idno type="pmid">18798692</idno><idno type="doi">10.1371/journal.pbio.0060226</idno><idno type="wicri:Area/PubMed/Corpus">001A66</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A66</idno><idno type="wicri:Area/PubMed/Curation">001A66</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001A66</idno><idno type="wicri:Area/PubMed/Checkpoint">001A32</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001A32</idno><idno type="wicri:Area/Ncbi/Merge">001D49</idno><idno type="wicri:Area/Ncbi/Curation">001D49</idno><idno type="wicri:Area/Ncbi/Checkpoint">001D49</idno><idno type="wicri:Area/Main/Merge">003104</idno><idno type="wicri:Area/Main/Curation">003031</idno><idno type="wicri:Area/Main/Exploration">003031</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum.</title><author><name sortKey="Knoops, Kevin" sort="Knoops, Kevin" uniqKey="Knoops K" first="Kèvin" last="Knoops">Kèvin Knoops</name><affiliation wicri:level="3"><nlm:affiliation>Section Electron Microscopy, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Section Electron Microscopy, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden</wicri:regionArea><placeName><settlement type="city">Leyde</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Kikkert, Marjolein" sort="Kikkert, Marjolein" uniqKey="Kikkert M" first="Marjolein" last="Kikkert">Marjolein Kikkert</name></author><author><name sortKey="Worm, Sjoerd H E Van Den" sort="Worm, Sjoerd H E Van Den" uniqKey="Worm S" first="Sjoerd H E Van Den" last="Worm">Sjoerd H E Van Den Worm</name></author><author><name sortKey="Zevenhoven Dobbe, Jessika C" sort="Zevenhoven Dobbe, Jessika C" uniqKey="Zevenhoven Dobbe J" first="Jessika C" last="Zevenhoven-Dobbe">Jessika C. Zevenhoven-Dobbe</name></author><author><name sortKey="Van Der Meer, Yvonne" sort="Van Der Meer, Yvonne" uniqKey="Van Der Meer Y" first="Yvonne" last="Van Der Meer">Yvonne Van Der Meer</name></author><author><name sortKey="Koster, Abraham J" sort="Koster, Abraham J" uniqKey="Koster A" first="Abraham J" last="Koster">Abraham J. Koster</name></author><author><name sortKey="Mommaas, A Mieke" sort="Mommaas, A Mieke" uniqKey="Mommaas A" first="A Mieke" last="Mommaas">A Mieke Mommaas</name></author><author><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J" last="Snijder">Eric J. Snijder</name></author></analytic><series><title level="j">PLoS biology</title><idno type="eISSN">1545-7885</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Cryoelectron Microscopy</term><term>Endoplasmic Reticulum (metabolism)</term><term>Endoplasmic Reticulum (ultrastructure)</term><term>Humans</term><term>Imaging, Three-Dimensional</term><term>Immunohistochemistry</term><term>Intracellular Membranes (metabolism)</term><term>Intracellular Membranes (ultrastructure)</term><term>Macromolecular Substances (metabolism)</term><term>Models, Biological</term><term>Protein Subunits (genetics)</term><term>Protein Subunits (metabolism)</term><term>RNA Replicase (genetics)</term><term>RNA Replicase (metabolism)</term><term>RNA, Double-Stranded (genetics)</term><term>RNA, Double-Stranded (metabolism)</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (metabolism)</term><term>SARS Virus (physiology)</term><term>SARS Virus (ultrastructure)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN double brin (génétique)</term><term>ARN double brin (métabolisme)</term><term>ARN viral (génétique)</term><term>ARN viral (métabolisme)</term><term>Animaux</term><term>Cryomicroscopie électronique</term><term>Humains</term><term>Imagerie tridimensionnelle</term><term>Immunohistochimie</term><term>Lignée cellulaire</term><term>Membranes intracellulaires (métabolisme)</term><term>Membranes intracellulaires (ultrastructure)</term><term>Modèles biologiques</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>RNA replicase (génétique)</term><term>RNA replicase (métabolisme)</term><term>Réplication virale</term><term>Réticulum endoplasmique (métabolisme)</term><term>Réticulum endoplasmique (ultrastructure)</term><term>Sous-unités de protéines (génétique)</term><term>Sous-unités de protéines (métabolisme)</term><term>Structures macromoléculaires (métabolisme)</term><term>Virus du SRAS (physiologie)</term><term>Virus du SRAS (ultrastructure)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Protein Subunits</term><term>RNA Replicase</term><term>RNA, Double-Stranded</term><term>RNA, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Macromolecular Substances</term><term>Protein Subunits</term><term>RNA Replicase</term><term>RNA, Double-Stranded</term><term>RNA, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN double brin</term><term>ARN viral</term><term>Protéines virales</term><term>RNA replicase</term><term>Sous-unités de protéines</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Endoplasmic Reticulum</term><term>Intracellular Membranes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN double brin</term><term>ARN viral</term><term>Membranes intracellulaires</term><term>Protéines virales</term><term>RNA replicase</term><term>Réticulum endoplasmique</term><term>Sous-unités de protéines</term><term>Structures macromoléculaires</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Endoplasmic Reticulum</term><term>Intracellular Membranes</term><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Cryoelectron Microscopy</term><term>Humans</term><term>Imaging, Three-Dimensional</term><term>Immunohistochemistry</term><term>Models, Biological</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term><term>Cryomicroscopie électronique</term><term>Humains</term><term>Imagerie tridimensionnelle</term><term>Immunohistochimie</term><term>Lignée cellulaire</term><term>Membranes intracellulaires</term><term>Modèles biologiques</term><term>Réplication virale</term><term>Réticulum endoplasmique</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Positive-strand RNA viruses, a large group including human pathogens such as SARS-coronavirus (SARS-CoV), replicate in the cytoplasm of infected host cells. Their replication complexes are commonly associated with modified host cell membranes. Membrane structures supporting viral RNA synthesis range from distinct spherular membrane invaginations to more elaborate webs of packed membranes and vesicles. Generally, their ultrastructure, morphogenesis, and exact role in viral replication remain to be defined. Poorly characterized double-membrane vesicles (DMVs) were previously implicated in SARS-CoV RNA synthesis. We have now applied electron tomography of cryofixed infected cells for the three-dimensional imaging of coronavirus-induced membrane alterations at high resolution. Our analysis defines a unique reticulovesicular network of modified endoplasmic reticulum that integrates convoluted membranes, numerous interconnected DMVs (diameter 200-300 nm), and "vesicle packets" apparently arising from DMV merger. The convoluted membranes were most abundantly immunolabeled for viral replicase subunits. However, double-stranded RNA, presumably revealing the site of viral RNA synthesis, mainly localized to the DMV interior. Since we could not discern a connection between DMV interior and cytosol, our analysis raises several questions about the mechanism of DMV formation and the actual site of SARS-CoV RNA synthesis. Our data document the extensive virus-induced reorganization of host cell membranes into a network that is used to organize viral replication and possibly hide replicating RNA from antiviral defense mechanisms. Together with biochemical studies of the viral enzyme complex, our ultrastructural description of this "replication network" will aid to further dissect the early stages of the coronavirus life cycle and its virus-host interactions.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country><region><li>Hollande-Méridionale</li></region><settlement><li>Leyde</li></settlement></list><tree><noCountry><name sortKey="Kikkert, Marjolein" sort="Kikkert, Marjolein" uniqKey="Kikkert M" first="Marjolein" last="Kikkert">Marjolein Kikkert</name><name sortKey="Koster, Abraham J" sort="Koster, Abraham J" uniqKey="Koster A" first="Abraham J" last="Koster">Abraham J. Koster</name><name sortKey="Mommaas, A Mieke" sort="Mommaas, A Mieke" uniqKey="Mommaas A" first="A Mieke" last="Mommaas">A Mieke Mommaas</name><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J" last="Snijder">Eric J. Snijder</name><name sortKey="Van Der Meer, Yvonne" sort="Van Der Meer, Yvonne" uniqKey="Van Der Meer Y" first="Yvonne" last="Van Der Meer">Yvonne Van Der Meer</name><name sortKey="Worm, Sjoerd H E Van Den" sort="Worm, Sjoerd H E Van Den" uniqKey="Worm S" first="Sjoerd H E Van Den" last="Worm">Sjoerd H E Van Den Worm</name><name sortKey="Zevenhoven Dobbe, Jessika C" sort="Zevenhoven Dobbe, Jessika C" uniqKey="Zevenhoven Dobbe J" first="Jessika C" last="Zevenhoven-Dobbe">Jessika C. Zevenhoven-Dobbe</name></noCountry><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="Knoops, Kevin" sort="Knoops, Kevin" uniqKey="Knoops K" first="Kèvin" last="Knoops">Kèvin Knoops</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003031 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003031 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:18798692
|texte= SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18798692" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |